Guanidine is a crystalline compound of strong alkalinity formed by the oxidation of guanine. It is used in the manufacture of plastics and explosives. It is found in urine as a normal product of protein metabolism. With a pKa of 12.5, guanidine is protonated, with a charge of +1 in physiological conditions.
Guanidine Hydrochloride has chaotropic properties and is used to denature proteins. Empirically, guanidine hydrochloride is known to denature proteins with a linear relationship between concentration and free energy of unfolding.
Guanidines are a group of organic compounds sharing a common functional group with the general structure (R1R2N)(R3R4N)C=N-R5. The central bond within this group is that of an imine; the other recognizable motif within this group is an aminal. An example of a guanidine is triazabicyclodecene.Guanidine is currently being considered as an alternative fuel. In the presence of a catalyst, a mole of free-base guanidine combines with 2 moles of water to form 3 moles of ammonia and 1 mole of carbon dioxide. The ammonia can be used directly as a fuel for internal combustion engines, or decomposed into nitrogen and hydrogen gas for use in fuel cells. The guanidine could be supplied as a fuel in solid form as pure guanidine (melting point ~ 50 C) or as a lower melting point eutectic mixture with urea. Guanidine could also be supplied as solutions in ethanol, as a replacement for the gasoline component in E85 fuel.
Guanidine, Hydrochloride
Guanidine Chloride;Guanidine Hydrochloride;Guanidinium chloride;Guanidine monohydrochloride;Guanidine HCl
MB Grade
(NH2)2C=NH•HCl
Formula Weight: 95.53
PRODUCT SPECIFICATIONS
Form: White/clear crystals
Identity: By IR
Assay: 99.5%
Solubility (6M, H2O): Clear and colorless
A (225 nm, 6M, 1 cm): 0.2
A (260 nm, 6M, 1 cm): 0.03
Melting Point: 184 ± 3°C
Insoluble Matter: Negligible
Residue on Ignition: 0.05%
Aldehyde: None detected
Ammeline: None detected
Arsenic (As): 1 ppm
Copper (Cu): 1 ppm
Iron (Fe): 2 ppm
Lead (Pb): 5 ppm
Sulfate (SO4): 50 ppm
Zinc (Zn): 1 ppm
DNase (endo): None detected
RNase: Passes test
Protease: None detected
Applications:
•Used for extraction of RNA.
•Preparation of high molecular weight genomic DNA.
•Solubilizing inclusion bodies.
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